NAD+ 500 mg reconstitution calculator
How much bac water for NAD+ 500 mg?
There’s no single right amount: the water sets the strength, not how much NAD+ is in the vial, so use the volume on your pharmacy’s instructions. For example, 5 mL makes 100 mg/mL and 10 mL makes 50 mg/mL; the table below shows what each volume means on a U-100 syringe.
Your NAD+ vial
Syringe
1 mL · 2-unit marks
Work backwards
Find the water for a given reading in units.
Enter your numbers to see the result.
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This is arithmetic, not medical advice. Confirm with your prescriber or pharmacist.
How our calculator worksAdvanced
Vial capacityOptional
The volume printed on the vial or its box, not the mg. We’ll tell you if the water won’t fit.
What each volume makes
| Water | Strength | Each unit | 1 mg is |
|---|---|---|---|
| 5 mL | 100 mg/mL | 1 mg | 1 unit |
| 10 mL | 50 mg/mL | 500 mcg | 2 units |
Examples; use the volume on your pharmacy’s instructions. Units are on a U-100 syringe: 100 units = 1 mL.
5 mL and 10 mL need a vial that holds at least that much. Check the size printed on your vial, in mL, before adding water (how much water a vial can hold).
About the 500 mg vial
500 mg takes milliliters of water
NAD+ vials hold hundreds of mg, so the example volumes here are 5 and 10 mL rather than the 1 to 3 mL used for most peptide vials. With 5 mL the vial is 100 mg/mL and each unit holds 1 mg; with 10 mL it’s 50 mg/mL and each unit holds 0.5 mg. A full 100-unit (1 mL) syringe holds 100 mg at 5 mL, or 50 mg at 10 mL. On the U-100 scale 5 mL is 500 units, five times what a 100-unit syringe holds, so ask your pharmacist what to add the water with. Both volumes are more than a 3 mL vial can take: check your vial’s size in mL first (how much water a vial can hold).
Category 1 isn't an approval
No NAD+ injection is FDA-approved. NAD is in Category 1 of the FDA’s interim 503A policy (list updated May 14, 2026): while the FDA evaluates it, the agency “does not intend to take action against a compounder” using it, provided the conditions in its guidance are met (FDA list, FDA). The exact form matters: the same list puts “Beta-Nicotinamide Adenine Dinucleotide Disodium Salt Trihydrate” in Category 3, which is “not eligible for the policy that applies to substances in category 1”. Category 1 says nothing about how much is in your vial, so enter the strength from your pharmacy’s label.
Food-grade NAD+ and the reactions the FDA has seen
The FDA has warned that some compounders used food-grade NAD+ sold by repackagers to make IV products. Food-grade ingredients aren’t suitable for sterile drugs without appropriate processing, “due to the high risk of contamination with microbes and endotoxins, which can harm patients” (FDA). The agency has received adverse event reports after NAD+ injections, “including severe chills, shaking, vomiting and fatigue with some requiring medical treatment”, which it says are consistent with excessive endotoxin levels. Ask your pharmacy where its NAD+ comes from, and tell your prescriber about any reaction like these.
Many draws from one vial: the 28-day guidance
If your vial holds multiple doses, the stopper gets punctured many times. The FDA generally recommends using multi-dose vials of sterile medicine within 28 days of first use “because there is an increased risk of contamination after 28 days”, and discarding the vial then “even if medication remains in the vial” (FDA). The calculator above shows how many doses of your prescribed amount the vial holds. If that count runs past 28 days, or past the beyond-use date on your pharmacy’s label, ask your pharmacist how to handle it before you mix.
Sources for these notes
- Bulk Drug Substances Nominated for Use in Compounding Under Section 503A of the FD&C Act (updated May 14, 2026)U.S. Food and Drug Administration, 2026 (opens in a new tab)
- Bulk Drug Substances Used in Compounding Under Section 503A of the FD&C ActU.S. Food and Drug Administration, 2026 (opens in a new tab)
- FDA reminds compounders to use ingredients suitable for sterile compoundingU.S. Food and Drug Administration, 2024 (opens in a new tab)
- FDA’s Concerns with Unapproved GLP-1 Drugs Used for Weight LossU.S. Food and Drug Administration, 2026 (opens in a new tab)
About NAD+
US regulatory status
Compounding restricted
No NAD+ injection is approved by the FDA. NAD is in Category 1 of the FDA’s interim 503A policy (list updated May 14, 2026), so state-licensed pharmacies may compound it under certain conditions while the FDA evaluates it; that is not an approval, and the FDA has warned compounders against making IV NAD+ from food-grade ingredients.
As of
Sources (3)
- Bulk Drug Substances Nominated for Use in Compounding Under Section 503A of the FD&C Act (updated May 14, 2026)U.S. Food and Drug Administration, 2026 (opens in a new tab)
- Bulk Drug Substances Used in Compounding Under Section 503A of the FD&C ActU.S. Food and Drug Administration, 2026 (opens in a new tab)
- FDA reminds compounders to use ingredients suitable for sterile compoundingU.S. Food and Drug Administration, 2024 (opens in a new tab)
How this NAD+ calculator works
This NAD+ reconstitution calculator starts from the 500 mg in your vial. Add the water you mix it with and the dose your prescriber gave you; it divides the mg by the mL to get the strength, then turns your dose into mL and units on a 30, 50 or 100-unit syringe.
Peptinote never suggests a dose or a water volume. The example volumes are there to make the math easy to check, not as advice. For the full method, see the peptide calculator.
Questions people ask
How much bacteriostatic water to mix with NAD+ 500mg?
There’s no single right amount: use the volume on your pharmacy’s instructions. The water only sets the strength; the vial still holds 500 mg however much you add. With 5 mL it’s 100 mg/mL (1 mg per unit) and with 10 mL 50 mg/mL (500 mcg per unit). Enter your volume and prescribed dose above to get the units. This product isn’t FDA-approved, so no official label sets a water volume and its contents aren’t verified.
How much bac water for 500mg vial of NAD?
Whatever volume you add, only the strength changes; the vial still holds 500 mg. More water spreads it out, so each unit holds less and the same dose takes more units: easier to measure, but more liquid to inject. Going from 5 mL to 10 mL takes each unit from 1 mg to 500 mcg. Check the volume on your pharmacy’s instructions first. This product isn’t FDA-approved, so no official label sets a water volume and its contents aren’t verified.
How much bac water for NAD+?
Use the volume your pharmacy specifies, and never more than the vial itself can hold (its size in mL, not the 500 mg on the label). If you weren’t given a volume, ask a pharmacist before mixing. For reference, the bac water calculator shows the arithmetic: water (mL) = vial mg × units ÷ (dose mg × 100). This product isn’t FDA-approved, so no official label sets a water volume and its contents aren’t verified.
How do I reconstitute 500 mg of NAD+?
Wipe both stoppers with alcohol and let them dry. Draw the volume of bacteriostatic water your pharmacy specifies into a new syringe, add it slowly down the inside of the vial, then swirl gently until clear; don’t shake. Label it with the strength: 500 mg in 5 mL is 100 mg/mL. Full steps: how to reconstitute peptides.
What are the steps to reconstitute a 500 mg NAD vial?
Mixing your NAD+ 500 mg vial takes the bacteriostatic water your pharmacy specifies and a new sterile syringe. Add the water slowly against the glass and swirl, never shake, until the liquid is clear. If it stays cloudy or has particles, don’t use it; call your pharmacy. Then write the strength and date on the vial and store it as the label says (how to store peptides).
Is there a peptide calculator for NAD+?
This page is the NAD+ 500 mg reconstitution calculator. Enter the water you add (or tap an example volume) and your prescribed dose, and it shows the units to draw on a 30, 50 or 100-unit syringe, the strength, and the doses in the vial, with every step of the math. It never suggests a dose.
How do NAD+ units convert to mg?
Units measure volume (1 unit = 0.01 mL), so converting them to mg needs the strength: mg = units ÷ 100 × mg/mL. In a 500 mg vial, with 5 mL each unit holds 1 mg and with 10 mL each unit holds 500 mcg. Multiply by the units you draw, or let the calculator above do it, with every step shown.
Can Peptinote tell me how many units to take?
No. Peptinote never suggests a dose. Enter the dose your prescriber gave you, and the calculator shows how to measure it: the units, the mL and where the line sits on your syringe, with every step of the math. If you aren’t sure what your dose is, ask your prescriber or pharmacist before drawing anything. How our calculator works.
Other NAD+ vial sizes
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Every result is plain arithmetic from formulas we publish in full and check with automated tests. Peptinote doesn’t suggest doses and never sells or links to sellers of peptides.